Comparative analysis of carrier systems for delivering bone morphogenetic proteins
정임희, 임현창, 이은웅, 이중석, 정의원, 최성호,
소속 상세정보
정임희 ( Jung Im-Hee ) - Yonsei University College of Dentistry Department of Periodontology
임현창 ( Lim Hyun-Chang ) - Kyung Hee University School of Dentistry Department of Periodontology
이은웅 ( Lee Eun-Ung ) - Yonsei University College of Dentistry Department of Periodontology
이중석 ( Lee Jung-Seok ) - Yonsei University College of Dentistry Department of Periodontology
정의원 ( Jung Ui-Won ) - Yonsei University College of Dentistry Department of Periodontology
최성호 ( Choi Seong-Ho ) - Yonsei University College of Dentistry Department of Periodontology
KMID : 0363020150450040136
Abstract
Purpose: The objective of this study was to comparatively assess the bone regenerative capacity of absorbable collagen sponge (ACS), biphasic calcium phosphate block (BCP) and collagenated biphasic calcium phosphate (CBCP) loaded with a low dose of recombinant human bone morphogenetic protein-2 (rhBMP-2).
Methods: The CBCP was characterized by X-ray diffraction and scanning electron microscopy. In rabbit calvaria, four circular 8-mm-diameter defects were created and assigned to one of four groups: (1) blood-filled group (control), (2) rhBMP-2-soaked absorbable collagen sponge (0.05 mg/mL, 0.1 mL; CS group), (3) rhBMP-2-loaded BCP (BCP group), or (4) rhBMP-2-loaded CBCP (CBCP group). The animals were sacrificed either 2 weeks or 8 weeks postoperatively. Histological and histomorphometric analyses were performed.
Results: The CBCP showed web-like collagen fibrils on and between particles. Greater dimensional stability was observed in the BCP and CBCP groups than in the control and the CS groups at 2 and 8 weeks. The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week. The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.
Conclusions: The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.
키워드
Bone morphogenetic protein 2 ; Bone regeneration ; Calcium phosphate ; Collagen
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